Bio-Speedy® SARS-CoV-2 N501Y Mutation Detection Kit
It is predicted that SARS-CoV-2 Variant of Concern 202012/01 (VOC-202012/01), which is estimated to have emerged in the UK in 2020, increased the transmission rate of SARS-CoV-2 by approximately 70%. The N501Y mutation, which is located in the receptor-binding motif (RBM) of the C-terminal domain (CTD) on the S gene and has been identified to increase binding affinity to the human ACE2 protein, is of particular importance among other mutations of VOC. All genomes containing N501Y sequenced in the UK belong to VOC-202012/01 since September 2020. The UK experience suggests that a large increase in SARS-CoV-2 positive cases is expected in the future if the amount of VOC-202012/01 detected is increasing despite its low prevalence. 77% of the sequenced SARS-CoV-2 genomes containing HV69-70del is VOC-202012/01. Therefore, screening only HV69-70del will not be decisive in regions where the VOC-202012/01 prevalence is low. The co-presence of N501Y and HV69-70del is a case only for VOC-202012/01 and can be used for the screening regardless of the variant's prevalence.
Controlling VOC-202012/01 carriers to access country borders, accelerating the filiation studies, and creating more specific quarantine conditions are possible by using Bio-Speedy® SARS-CoV-2 N501Y Mutation Detection Kit, which can give results less than 30 minutes.
Bio-Speedy® SARS-CoV-2 N501Y Mutation Detection Kit is a one-step reverse transcription and real-time PCR (RT-qPCR) for rapid screening of VOC-202012/01 specific Spike N501Y and HV69-70del mutations in SARS-CoV-2 positive samples.
The Bio-Speedy® SARS-CoV-2 N501Y Mutation Detection Kit allows detection of both VOC-202012/01 (N501Y and HV69-70del positive cases) and the suspected cases of the 501Y.V2 and P.1 variants (N501Y positive and HV69-70del negative cases) with considerably lower time and cost compared to the sequencing-based technologies.
250, 500, 1000 reactions
BS-N501Y-250, BS-N501Y-500, BS-N501Y-1000
The strip/plate mentioned below is suitable for use with Bio-Rad CFX96 TouchTM Real-Time PCR instrument. You can contact us for product information.
Cat#: BS2001 Ultra optical half skirted qPCR plate and seal (96 well)
Cat#: BS1001; Ultra Optical qPCR Strip and Cap (8 well)