The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a new, rapidly spreading human beta coronavirus. It was first identified in Wuhan and caused a disease named Coronavirus Disease 2019 (COVID-19). Infection by SARS-CoV-2 causes a respiratory illness that varies in severity from mild upper respiratory symptoms to severe progressive respiratory failure that requires intensive care and may lead to death. SARS-CoV-2 is a positive-sense, single-stranded RNA (ssRNA), group IV virus. The ∼30 k base-pair genome is highly similar to the human SARS-CoV and bat CoV-SARS-like genomes. The virus binds to an angiotensin-converting enzyme 2 (ACE2) receptor for cell entry.
SARS-CoV-2 is the seventh coronavirus identified that is known to infect humans (HCoV). Four of these viruses, HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43, are endemic, seasonal and tend to cause mild respiratory disease. The other two viruses are the more virulent zoonotic Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus type 1 (SARS-CoV-1).
Diagnostic testing can involve detecting the virus itself (viral RNA or antigen) or detecting the human immune response to infection (antibodies or other biomarkers). The most widely used molecular method approved by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) to detect SARS-CoV-2 is the real-time reverse transcription polymerase chain reaction (RT-qPCR).
Bio-Speedy® SARS-CoV-2 detection kits are used for the in vitro qualitative detection of RNA from the epidemic virus SARS-CoV-2 in different human samples including nasopharyngeal swab (NPS), oropharyngeal swab (OPS), bronchoalveolar lavage (BAL), nasopharyngeal aspirate (NPA), sputum, saliva, and gargle from patients suspected of COVID-19 by a healthcare provider or for screening of individuals without symptoms or other reasons to suspect COVID-19.
The Bio-Speedy® SARS-CoV-2 detection kits test and distinguish SARS-CoV-2 in a single multiplex RT-qPCR reaction in less than 30 minutes.